Gülbübü KURMANBEKOVA | Yılmaz KAYA

Article | 2024 | Bioremediation Journal ( Published online: 27 Feb 2024 )

There are mounting concerns over the high concentrations of non-biogenic, toxic halogenated organic compounds being liberated into the ecosystem. Therefore, this study’s isolation of a novel bacterium from a contaminated stream in Fatsa, Ordu, Turkey, adept in degrading 2,2-dichloropropionic (of 2,2-DCP) is a welcome endeavor. The ability of the bacterial isolate to utilize 2,2-DCP as the sole carbon and energy source was discovered when the bacterium was observed to grow well on liquid minimal media containing 20 mM of 2,2-DCP, showing a doubling time of 14.2 h. The following genetic and biochemical characterizations revealed that . . .the 16S rRNA sequence of the fatsa001strain is identical (99) to Pseudomonas fluorescence, after which the sequence was deposited in the NCBI GenBank as Pseudomonas sp. strain fatsa001 (MN098848). The halogen-degrading ability of the P. fluorescens fatsa001 bacterium was again confirmed by the PCR data, which showed the presence of a conserved group of amino acids from the group I dehalogenase gene. It worth mentioning here that this is the first report on a P. fluorescence bacterial strain with the ability to degrade toxic 2,2-DCP. The detoxification ability of this bacterium envisages its practicality as an in situ environmental bioremediation agent. Keyword: dalapon herbicide; dehalogenase; dehalogenation; recalcitran More less

Cumagül Nurakun Kızı | Rita İSMAİLOVA

Article | 2018 | Interactive Learning Environments26 ( 8 )

In this paper, the determinants of the intention to use online learning management system (LMS) among students in one of universities in the Kyrgyz Republic were studied, using quantitative and qualitative analysis. A total of 541 responses from students, enrolled in online courses during the fall and spring semesters 2016–2017, were used to examine students attitude toward online education. In addition, interviews with six instructors were conducted. Results showed that success of students in e-courses depend on year of education and the academic major of students. Factors, defined in the scope of this work, namely, technical chara . . .cteristics of LMS, ease of use, feedback options of LMS and advantages of LMS use, were also found to be significant for students in their success in online courses. In interviews with instructors, three main points were highlighted, namely, problem with students’ perception of online courses, lack of online education experience among instructors and administrative issue More less

Fatih HATİPOĞLU

Article | 2021 | British Poultry Science63 ( 3 )

1. Aflatoxins (AFs) are metabolites which especially have toxic effects on proteins, and are detoxified by the aflatoxin-B-1 aldehyde reductase (AFAR) pathway. In this pathway, the aldo-keto reductase family 7, member A2 (AKR7A2) enzyme, which is controlled by nucleic-related erythroid factor 2 (Nrf2), plays an active role. However, data on the efficacy of this critical pathway in broilers is limited. 2. The aim of the following study was to investigate the changes in the expression levels of AKR7A2, Nrf2, and caspase-3, and the effects of Nigella sativa seeds (NS), thymoquinone (TMQ), and bentonite (BNT) in broilers exposed to AFs. . . . 3. One-hundred broilers were divided into ten groups (control (CNT); AF; NS; TMQ; BNT; AF+TMQ; AF+NS; AF+BNT; AF+BNT+NS; AF+BNT+TMQ) and fed for 28 d. AF, TMQ, NS and BNT were added to diets at levels of 2 mg/kg, 300 mg/kg, 50 g/kg and 10 g/kg respectively. 4. The addition of AF to the diet decreased AKR7A2 and Nrf2 levels dramatically, but increased caspase-3 (P < 0.01). TMQ, NS and BNT additions to the diet eliminated all negative effects caused by AF (P < 0.01); and AKR7A2 and Nrf2 were further raised in TMQ and NS groups when compared to the control group. TMQ and NS showed a positive effect on detoxification parameters when given together with BNT. 5. Supplementation with NS and TMQ enhanced AF detoxification via the AFAR pathway, by increasing AKR7A2 and Nrf2 levels, in addition to reducing hepatocyte apoptosis More less

İbrahim İlker ÖZYİĞİT

Article | 2021 | International Journal of Phytoremediation23 ( 12 )

In this study, to determine whether having potential to be used as hyperaccumulator for Cd and Ni, numerous experiments were designed for conducting assessments for physiological and genotoxic changes along with defining possible alterations on mineral nutrient status of Lemna minor L. by applying Cd-Ni binary treatments (0, 100, 200 and 400 mu M). Our study revealed that there were increases in the concentrations of B, Cr, Fe, K, Mg, and Mn whereas decreases were noticed in the concentrations of Na and Zn and the levels of Ca were inversely proportional to Cd-Ni applications showing tendency to increase at the low concentration and . . . to decrease at the high concentration. Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) analyses revealed that rather than band losses and new band formations, mostly intensity changes in the band profiles, and low polymorphism and high genomic template stability (GTS) were observed. Although, to date, L. minor was defined as an efficient hyperaccumulator/potential accumulator or competent phytoremedial agent by researchers. Our research revealed that L. minor showing high accumulation capability for Cd and having low polymorphism rate and high genomic template stability is a versatile hyperaccumulator, especially for Cd; therefore, highly recommended by us for decontamination of water polluted with Cd. NOVELTY STATEMENT Many studies have been focused on the effects of individual metal ions. However, heavy metal contaminants usually exist as their mixtures in natural aquatic environments. Especially, Cd and Ni coexist in industrial wastes. In this study, the accumulation properties of Lemna minor for both Cd and Ni were investigated and the effects of Cd and Ni on the bioaccumulation of B, Ca, Cu, Fe, Mg, K, Mn, Na, Pb and Zn in L. minor were also determined. This study furthermore aimed to assess the genotoxic effects of Cd and Ni found in being extended concentrations on DNA using the Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) method More less

Hakan ÇETİNTAŞ

Article | 2016 | Energy Sources, Part B: Economics, Planning, and Policy11 ( 3 )

This study investigates the causality relationship between energy consumption and economic growth in 17 transition countries, which are Albania, Armenia, Belarus, Bulgaria, Czech Republic, Estonia, Hungary, Kazakhstan, Kyrgyz Republic, Latvia, Moldova, Poland, Romania, Russian Federation, Slovenia, Ukraine, and Georgia. Empirical findings indicate that there is unidirectional causality from economic growth to energy consumption in the long run. The results support for conservation hypothesis suggests that energy conservation policies have no effect on economic growth. They can simultaneously achieve policy goals concerning growth and energy.

İbrahim İlker ÖZYİĞİT

Article | 2019 | Biotechnology and Biotechnological Equipment33 ( 1 )

Although stresses induce generation of reactive oxygen species (ROS), which are highly reactive and toxic, and cause severe damage to cellular components; plants have very efficient enzymatic ROS-scavenging mechanisms. Despite the substantial knowledge produced about these enzymes, we still have limited knowledge regarding their expression patterns in relation to the stress type, duration and strength. Thus, taking advantage of microarray data, this work evaluated the abiotic stresses (salt, cold, heat and light) induced regulation of six antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), gl . . .utathione peroxidase (GPX), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR), in 10 natural Arabidopsis ecotypes. The expression profiles of 36 genes encoding six enzymatic antioxidants including CSD1-3, FSD1-3, MSD1-2, CAT1-3, APX1-6, APXT, APXS, GPX1-8, MDAR1-5 and DHAR1-4 were investigated. In particular, FSD1, FSD2, CSD1 and CSD2 genes coding for SOD; CAT2 and CAT3 for CAT; APX3-6, APXT and APXS for APX; GPX1, GPX2, GPX5, GPX6 and GPX7 for GPX; MDAR2-4 for MDHAR; and DHAR1 and DHAR3 for DHAR families appeared to be more differentially expressed under given stress conditions. Primarily, high light as well as salt and cold stresses considerably up-regulated the gene expression, whereas cold stress significantly led to the down-regulation of genes. The overall expression pattern of ecotypes suggested that the studied Arabidopsis genotypes had different stress tolerance More less

İbrahim İlker ÖZYİĞİT | Bermet KIDIRALİYEVA | Kadırbay ÇEKİROV | Gülbübü KURMANBEKOVA | Nurzat ŞAYKİYEVA

Article | 2021 | Journal of Radiation Research and Applied Sciences14 ( 1 )

Kyrgyzstan still suffers from the past practices of radioactive industry operated by the Soviet Union that caused significant impact on ecosystems in this region, especially related with storage of uranium in inadequate conditions. There are at least 50 abandoned sites used as radioactive waste dumps in the country. Due to the pressure of natural and anthropogenic reasons, the structural foundations of mine waste dumps are gradually losing their integrities. Here, particular interest of our research is to reveal current state of radioactive contamination and to make evaluation on the radiological impacts of pollution caused by urani . . .um mine waste dump, one is situated near Kaji-Say Village in Issyk Kul Region-Kyrgyzstan. In this study, the leaf, stem, and root parts of Perovskia abrotanoides Kar. and their co-located soils as study materials collected from five different localities were used for investigation of existent alterations on element uptake and genetic material in the plant using ICP-MS and ISSR marker technique. Also, radioactivity readings were recorded using Geiger counter. The data showed that the levels of radiation (in mR/h) were found to be significantly high in comparison with normal acceptable limits. Uptake patterns of certain elements in P. abrotanoides grown in the uranium waste dump site were modified extensively as reductions and increments due to strong radioactive leakage in comparison with the control. Also, the results indicated that changes in ISSR profiles from exposed plant leaf samples included variation in band intensities, losses of normal bands, and the appearances of new bands compared to unexposed (control) plant leaf sample. Considering people living around the area where the research was conducted, the consequences of contamination as our data suggest could cause health problems through radioactive leakage More less

Gülzada STANALİYEVA | Bakıtbek ORUNBEKOV

Article | 2019 | Annals of the International Communication Association43 ( 3 )

Methodological issues abound when conducting cross-cultural research. In this manuscript we discuss three methodological issues present in many cross-cultural communication studies: lack of geographic diversity, reliability, and validity. To explore these issues the Personal Report of Communication Apprehension (PRCA-24) is assessed. The PRCA-24 serves as an exemplar of a US-designed and -validated measure frequently used outside the US without tests of validity or measurement invariance. In fact, since 1990 less than 10 studies have reported validity results, often citing fit issues. The PRCA-24 was administered to respondents from . . . 11 countries and failed to yield acceptable fit statistics in all samples, showing poor construct validity. Implications for cross-cultural research are discussed, with particular emphasis on recommendations for increased cross-cultural methodological rigo More less

Mehmet BALCILAR

Proceedings Paper | 2004 | Emerging Markets Finance and Trade40 ( 5 )

This paper examines persistence in Turkish inflation rates using data from consumer and wholesale price indices. The inflationary process in Turkey is believed to be highly inertial, which should lead to strongly persistent inflation series. Persistence of seventy-five inflation series at various aggregation levels is examined by estimating models that allow long memory through fractional differencing. The order of fractional differencing is estimated using several semiparametric and maximum likelihood methods. Persistence of each series is evaluated using the time required for a given percentage of the effect of a shock to dissipat . . .e. We find that disaggregate inflation series show no significant persistence. We found that only twelve out of seventy-five series require more than six months for 99 percent of the effect of a shock to dissipate. Thus, the paper finds evidence of spurious long memory due to aggregation. - Keywords: aggregation, fractional differencing, inflation, inertia, long memory models, persistenc More less

Ali Osman SOLAK

Article | 2011 | Instrumentation Science & Technology39 ( 2 )

Benzo[c]cinnoline (BCC) molecules were electrochemically grafted onto a glassy carbon (GC) surface in nonaqueous media, and the modified surface was characterized using cyclic voltammetry (CV) with redox probes. Blockage of the electron transfer on the modified surface was observed using redox probes. Electrocatalytic effect of 2BCC modified GC (2BCC-GC) electrode surface towards to the electrochemical reduction of dioxygen was also investigated. A mechanistic scheme for the electrochemical catalysis was proposed. To clarify the mechanism of the dioxygen reduction, a less basic film of 2-benzo[c]cinnoline 6-oxide (2BCCNO) molecules . . .was also prepared at the glassy carbon surface. The effect of electrochemical catalysis of dioxygen reduction at the 2BCC-GC surface was compared to that at the 2BCCNO-GC surface. - Keywords: 2-benzo[c]cinnoline modified electrode, benzo[c]cinnoline, cyclic voltammetry electrocatalytic dioxygen reduction, electrochemical modificatio More less

İbrahim İlker ÖZYİĞİT

Article | 2020 | Biotechnology and Biotechnological Equipment34 ( 1 )

Boron (B) is an essential plant micronutrient but studies regarding its transport are still limited to a few plants. This work identified two major B transport sequences in plants, NIP5;1 boric acid channel protein and BOR1 transporter. 80 BOR1 and 34 NIP5;1 homologs were identified in 18 different plant genomes. BOR1 homologs had a HCO3-transporter domain, 649-737 amino-acid residues with mainly basic nature, putative 8-11 transmembrane domains (TMDs) and 11-13 exons. NIP5;1 homologs had a MIP family domain, 294-311 amino-acid residues with basic nature, 5-6 putative TMDs and 3-5 exons. Tyrosine-based motif, acidic di-leucine motif . . . and lysine residue, reported for polarity, vacuolar sorting and B-dependent degradation, were identified in BOR1 homologs. Two NPA motifs and an ar/R selectivity filter with AIGR residues, reportedly essential in B transport, were also found in NIP5;1 homologs. Two NPA motifs in AtNIP5;1 and OsNIP3;1 homologs were NPS and NPV, whereas in sequences homologous to AtNIP6;1 were NPA/V. Besides, ar/R selectivity filters were identified with A(N/S/T)IGR residues in NIP5;1 and NIP3;1 homologs. The BOR1 and NIP5;1 model structures were mainly conserved. Under different perturbations, Arabidopsis thaliana NIP5;1 and NIP6;1 genes demonstrated similar expression patterns although they act in different tissues, suggesting a common regulatory mechanism, whereas BOR1 showed a different expression pattern. BOR1 was substantially expressed in primary root, radicle and flower; NIP5;1 in primary root and roots, and NIP6;1 in petiole. NIP5;1, 6;1 and BOR1 expression in other plant organs implied their involvement in different pathways in addition to B uptake and its mobilization More less

İlhan DOĞAN

Article | 2016 | Biotechnology and Biotechnological Equipment30 ( 2 )

Plants can be used as biological indicators in assessing the damage done by bioaccumulation of heavy metals and their negative impact on the environment. In the present research, Roman nettle (Urtica pilulifera L.) was employed as a bioindicator for cadmium (Cd) pollution. The comparisons between unexposed and exposed plant samples revealed inhibition of the root growth (∼25.96% and ∼45.92% after treatment with 100 and 200 µmol/L Cd concentrations, respectively), reduction in the total soluble protein quantities (∼53.92% and ∼66.29% after treatment with 100 and 200 µmol/L Cd concentrations, respectively) and a gradual genomic instab . . .ility when the Cd concentrations were increased. The results indicated that alterations in randomly amplified polymorphic DNA (RAPD) profiles, following the Cd treatments, included normal band losses and emergence of new bands, when compared to the controls. Also, the obtained data from F1 plants, utilized for analysis of genotoxicity, revealed that DNA alterations, occurring in parent plants due to Cd pollution, were transmitted to the next generation. - Keywords: RAPD-PCR, genotoxicity, cadmium (Cd), Roman nettle, heavy metal More less