İsmail ERPER

Article | 2022 | Egyptian Journal of Biological Pest Control32 ( 1 )

Background Entomopathogenic fungi are a potential biological control agent for many pests. In this study, 14 native Beauveria bassiana isolates were molecularly identified and assessed for their virulence and mortality against adults of the polyphagous planthopper Orosanga japonica, Melichar (Hemiptera: Ricaniidae) a polyphagous sap-feeding insect, under laboratory conditions. Results Isolates obtained from naturally infected adults O. japonica were molecularly identified as B. bassiana by sequencing the internal transcribed spacer and 26S large subunit of ribosomal DNA. In the bioassay, the radial growth and sporulation of isolates . . . significantly differed (P < 0.05). Concentration-time bioassays demonstrated that all isolates had a lethal effect on adult O. japonica at the concentration of 1 x 10(8) conidia ml(-1). The lethal times LT50 and LT90 values for each B. bassiana isolate, which indicate the time required to kill O. japonica, ranged between 2.44-3.19 and 3.78-5.01 days, respectively, at 20 degrees C, while their values were 1.74-2.76 and 2.76-4.10 days, respectively, at 25 degrees C depending on the native isolates. The concentration-mortality response showed a statistically significant difference between some isolates B. bassiana tested against adult O. japonica individuals (P < 0.05). With an LC50 value of 2.29 x 10(6) conidia ml(-1), the lowest effective EPF isolate for O. japonica was KA-78-14. The isolates caused 100 adult mortality in O. japonica within five days, while the mortality in the control group was less than 10. Conclusions These findings suggest that some B. bassiana isolates were more virulent on O. japonica and may play an important role in the biocontrol of O. japonica in Turkey More less

İsmail ERPER

Article | 2020 | Egyptian Journal of Biological Pest Control30 ( 1 )

The efficacy of the two entomopathogenic fungi, Metarhizium anisopliae (TR 106) and Beauveria bassiana (TR 217), was tested against the adults of the cowpea weevil, Callosobruchus maculatus F. (Coleoptera: Chrysomelidae: Bruchinae) in laboratory. Two concentrations of conidial suspensions (1 x 10(6) and 1 x 10(8) conidia/ml) of M. anisopliae and B. bassiana isolates were directly applied on four pairs of adult beetles in Petri dishes (2 ml/dish) and then incubated at two different climatic regimes; 22 +/- 1 degrees C and 26 +/- 1 degrees C with 70 +/- 5% RH, 16:8 h light-to-dark. Lethal time values (LT50 and LT90) were calculated us . . .ing probit analysis. As a result, the LT50 and LT90 values of M. anisopliae (1 x 10(8) conidia/ml) were 4.45 and 5.34 days at 26 degrees C and 5.17 and 6.15 days at 22 degrees C, respectively. LT50 and LT90 values of B. bassiana (1 x 10(8) conidia/ml) were 4.07 and 5.11 days at 26 degrees C and 4.07 and 5.41 days at 22 degrees C, respectively. LT50 and LT90 values of M. anisopliae (1 x 10(6) conidia/ml) were 5.42 and 6.43 days at 26 degrees C and 6.08 and 7.54 days at 22 degrees C, respectively. The LT50 and LT90 values of B. bassiana (1 x 10(6) conidia/ml) were 5.67 and 7.15 days at 26 degrees C and 5.47 and 7.50 days at 22 degrees C, respectively. Approximately 100% of mycoses were obtained in all treatments. In general, the effectiveness of these two entomopathogens increased by increasing suspension concentrations and temperature. These results suggest that the two isolates may be very successful in biological control of the C. maculatus and may be alternatives for chemical pest management More less

İlker KEPENEKÇİ

Article | 2016 | Egyptian Journal of Biological Pest Control26 ( 1 )

İlker KEPENEKÇİ

Article | 2016 | Egyptian Journal of Biological Pest Control26 ( 1 )

Potential of three native Turkish isolates of entomopathogenic nematodes (EPNs) (Steinernema feltiae, S. carpocapsae and Heterorhabditis bacteriophora) was evaluated against Holotrichapion pullum (Gyllenhal) (Coleoptera, Apionidae) under laboratory conditions. Suspensions of EPNs were applied at three concentrations (500, 1000 and 2000 IJs ml-1) and at two temperatures (15 and 20°C). After one week, mortality rate of H. pullum adults was assessed. Mortality rate was higher in the nematode treatments than in the control. All nematodes showed more effect depending on temperature increase. At 20°C and all concentrations, S. carpocapsae . . . was the most effect by (80.43, 83.35 and 82.15% mortality, respectively). S. feltiae had relatively high effects (30.44, 41.93 and 35.21% mortality, respectively), while H. bacteriophora had the lowest rates (24.15, 27.16 and 30.55% mortality, respectively). At 15°C, EPNs showed an effect less than 25%. Obtained results indicated that H. pullum adults are susceptible to EPNs infection in particular for S. carpocapsae (the black sea strain). Further studies are required under laboratory and field conditions. – Key words: Entomopathogenic nematodes, effects, Holotrichapion pullum, adults More less

İlker KEPENEKÇİ

Article | 2016 | Egyptian Journal of Biological Pest Control26 ( 1 )

The gen us of roo t lesio n nematod es (RLN), Pratylench us, feeds an d reprodu ces in the roo t co rtex of man y plant s pecies. RLN was rep roduced on carrot cu lture by using a pu re cu lture. In vitro tes ts were carried out to determine the effects of plant extracts from three ind igenous plants: h enbane, Hyoscyamus niger; b ead-tree, Melia a zedarah and commo n cocklebu r, Xanth ium strumarium on P. tho rn ei (the major wh eat root-les ion nematode). The nematodes were expos ed for 12, 24, 48, 72 an d 96 hrs in three concen tratio ns of plant extracts. Th e effect of plant extracts was perfo rmed usin g the sus pensions of P. . . . th ornei (100 nematodes ml-1) in dis tilled water. Distilled water was us ed as a co ntrol. All dis hes were kept at 28±2°C. The plant extracts of H. niger, M. azed arah and X. strumarium exh ibited hig hly p romising mortality rate (100%) after 72 hou rs of exposu re time. The res ult of this stud y s ugg ests the s tudied plant extracts to b e used for reducing the P. tho rn ei popu lation. – Key words: Root lesion nematode, Pratylenchus thornei, plant extracts, mortality More less