Tinatin DOOLOTKELDİEVA

Article | 2009 | Microbiology Insights ( 2 )

During this work we detected 5 species of pathogenic fungi that damage seedlings and saplings of the Tiang-Shang spruce fir, which cause a perceptible damage to the coniferous nurseries of Northern Kyrgyzstan and require activities to protect the woods. The biological activity of Streptomyces metabolites for raising the resistance of seedlings to phytopathogenic fungi was tested in laboratory and field conditions during three seasons. In order to evaluate the biological activity of Streptomyces preparations as biological agents, we have soaked the seeds in the suspension of Streptomyces metabolites with the concentrations of 50, 100 . . ., 500 mg per liter. In other variant, the seeds were at first processed in the culture of pathogenic fungi as infectious drowning of sprouts (Fusarium, Alternaria), crown rot of sprouts (Sclerotinia graminearum Elen.) and grey dew of needles (Hypodermella sulsigena Tub.), then they were treated in the suspension of Streptomyces. Also we have used a spraying treatment with the suspension of Streptomyces metabolites of the one-year-old seedlings of Tiang-Shang spruce fir already staggered and dying from infectious drowning. The best results against the Alternaria culture were demonstrated by the use of preparation S. griseogromogenes 2ч-8 at the concentration of 500 mgs per liter, which provided the safety of sprouts to 80.2%. Preparations of S. rubrogriseus ТК2-5, S. wistariopsis СП3-13 and S. griseogromogenes 2ч-8 were effective in protection of sprouts from Fusarium sp. cultures. They provided safety of sprouts to 80% on average More less

Tinatin DOOLOTKELDİEVA

Article | 2010 | Microbiology Insights ( 3 )

Wheat grain and its products are widely consumed as fodder and basic daily food stuffs in Kyrgyzstan. Mycobiota is known to produce hazardous effects to a consumer since it produces mycotoxins. Henceforth, mycobiota starting from the field stage to flour, grain and flour samples were selected for mycological analysis from eight sites of flour manufacture: grain stored in storehouses before milling, mechanically cleaned grain, washed grain, grain dried and prepared for mill, roughly-milled flour, first grade flour and high grade flour. The samples were analyzed using classical mycological and immunoassay methods in order to detect my . . .cotoxins producing fungi species. We isolated overall 27 species belonging to 7 genera. Mycotoxins producing species like Aspergillus flavus, Aspergillus ochraceus and Penicillium cyclopium were detected in the stored grains and in mechanically-cleaned grains. The species of Penicillium, Alternaria and Fusarium genera dominated in roughly-milled flour samples, so this site of flour manufacture still has a risk and danger of contamination with mycotoxins producing fungus. Only the final product i.e. the high grade flour lacked any fungal contamination. We recommend to scrutinize flour samples at the last stages of processing, particularly in the mills like В1, С1 and С4 More less

Tinatin DOOLOTKELDİEVA | Saykal BOBUŞOVA

Article | 2011 | Microbiology Insights ( 4 )

Background: In this study, wild-type fungal isolates, producing highly effective cellulolytic enzymes were selected for bioconversion of residues and waste from agriculture and rational utilization of energy resources for food production. Methods: We screened wild-type fungal isolates of Aspergillus, Penicillium, and Trichoderma with an enhanced ability to produce extracellular cellulase. We carried out solid-state fermentation on a medium of agricultural waste products, including wheat bran, beet peels, and cotton oil cake, as well as additional sources of nitrogen and mineral elements. Enzyme production by the fungal isolates was . . .detected within 14 days of cultivation. Results: Of 17 strains of Trichoderma, Aspergillus, and Penicillium tested, we identified Penicillium strain K-2-25 and Trichoderma lignorium strain T-22 to have high cellulolytic activity. K-2-25 demonstrated the highest activity after 48 hours of cultivation. T-22 also showed significant cellulolytic activity. Penicillium strain K-2-25 showed cellulolytic activity for 98–270 hours during cultivation, and the amount of reduced glucose was 945 mg. T. lignorium T-22 was the second most active strain, with glucose reduction of 835 mg. Conclusion: The strains K-2-25 and T-22 will are be recommended for biotechnological applications, especially for bioconversion of poor hardly decomposable vegetable waste products, such as like straw, into useful biomass. More less