İbrahim İlker ÖZYİĞİT

Article | 2021 | International Journal of Phytoremediation23 ( 12 )

In this study, to determine whether having potential to be used as hyperaccumulator for Cd and Ni, numerous experiments were designed for conducting assessments for physiological and genotoxic changes along with defining possible alterations on mineral nutrient status of Lemna minor L. by applying Cd-Ni binary treatments (0, 100, 200 and 400 mu M). Our study revealed that there were increases in the concentrations of B, Cr, Fe, K, Mg, and Mn whereas decreases were noticed in the concentrations of Na and Zn and the levels of Ca were inversely proportional to Cd-Ni applications showing tendency to increase at the low concentration and . . . to decrease at the high concentration. Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) analyses revealed that rather than band losses and new band formations, mostly intensity changes in the band profiles, and low polymorphism and high genomic template stability (GTS) were observed. Although, to date, L. minor was defined as an efficient hyperaccumulator/potential accumulator or competent phytoremedial agent by researchers. Our research revealed that L. minor showing high accumulation capability for Cd and having low polymorphism rate and high genomic template stability is a versatile hyperaccumulator, especially for Cd; therefore, highly recommended by us for decontamination of water polluted with Cd. NOVELTY STATEMENT Many studies have been focused on the effects of individual metal ions. However, heavy metal contaminants usually exist as their mixtures in natural aquatic environments. Especially, Cd and Ni coexist in industrial wastes. In this study, the accumulation properties of Lemna minor for both Cd and Ni were investigated and the effects of Cd and Ni on the bioaccumulation of B, Ca, Cu, Fe, Mg, K, Mn, Na, Pb and Zn in L. minor were also determined. This study furthermore aimed to assess the genotoxic effects of Cd and Ni found in being extended concentrations on DNA using the Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) method More less

İbrahim İlker ÖZYİĞİT

Article | 2019 | Biotechnology and Biotechnological Equipment33 ( 1 )

Although stresses induce generation of reactive oxygen species (ROS), which are highly reactive and toxic, and cause severe damage to cellular components; plants have very efficient enzymatic ROS-scavenging mechanisms. Despite the substantial knowledge produced about these enzymes, we still have limited knowledge regarding their expression patterns in relation to the stress type, duration and strength. Thus, taking advantage of microarray data, this work evaluated the abiotic stresses (salt, cold, heat and light) induced regulation of six antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), gl . . .utathione peroxidase (GPX), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR), in 10 natural Arabidopsis ecotypes. The expression profiles of 36 genes encoding six enzymatic antioxidants including CSD1-3, FSD1-3, MSD1-2, CAT1-3, APX1-6, APXT, APXS, GPX1-8, MDAR1-5 and DHAR1-4 were investigated. In particular, FSD1, FSD2, CSD1 and CSD2 genes coding for SOD; CAT2 and CAT3 for CAT; APX3-6, APXT and APXS for APX; GPX1, GPX2, GPX5, GPX6 and GPX7 for GPX; MDAR2-4 for MDHAR; and DHAR1 and DHAR3 for DHAR families appeared to be more differentially expressed under given stress conditions. Primarily, high light as well as salt and cold stresses considerably up-regulated the gene expression, whereas cold stress significantly led to the down-regulation of genes. The overall expression pattern of ecotypes suggested that the studied Arabidopsis genotypes had different stress tolerance More less

İbrahim İlker ÖZYİĞİT | Bermet KIDIRALİYEVA | Kadırbay ÇEKİROV | Gülbübü KURMANBEKOVA | Nurzat ŞAYKİYEVA

Article | 2021 | Journal of Radiation Research and Applied Sciences14 ( 1 )

Kyrgyzstan still suffers from the past practices of radioactive industry operated by the Soviet Union that caused significant impact on ecosystems in this region, especially related with storage of uranium in inadequate conditions. There are at least 50 abandoned sites used as radioactive waste dumps in the country. Due to the pressure of natural and anthropogenic reasons, the structural foundations of mine waste dumps are gradually losing their integrities. Here, particular interest of our research is to reveal current state of radioactive contamination and to make evaluation on the radiological impacts of pollution caused by urani . . .um mine waste dump, one is situated near Kaji-Say Village in Issyk Kul Region-Kyrgyzstan. In this study, the leaf, stem, and root parts of Perovskia abrotanoides Kar. and their co-located soils as study materials collected from five different localities were used for investigation of existent alterations on element uptake and genetic material in the plant using ICP-MS and ISSR marker technique. Also, radioactivity readings were recorded using Geiger counter. The data showed that the levels of radiation (in mR/h) were found to be significantly high in comparison with normal acceptable limits. Uptake patterns of certain elements in P. abrotanoides grown in the uranium waste dump site were modified extensively as reductions and increments due to strong radioactive leakage in comparison with the control. Also, the results indicated that changes in ISSR profiles from exposed plant leaf samples included variation in band intensities, losses of normal bands, and the appearances of new bands compared to unexposed (control) plant leaf sample. Considering people living around the area where the research was conducted, the consequences of contamination as our data suggest could cause health problems through radioactive leakage More less

İbrahim İlker ÖZYİĞİT

Article | 2019 | Allelopathy Journal48 ( 2 )

In Laboratory bioassays and Pot culture, we determined the effects of aqueous extracts of 7-allelopathic crops [Alfalfa (Medicago sativa L.), Sorghum (Sorghum bicolor L.), Sunflower (Helianthus annuus L.), Rape (Brassica napus L.), Rye (Secale cereal L.), Barley (Hordeum vulgare L) and Oat (Avena saliva L)] on the seedling growth of weed Hypericum triquetrifolium. In first Lab. bioassay, barley and rape were found most phytotoxic to Hypericum seedlings growth. Then these two crops effects were further tested in pot experiments, with different residues rates (R1, R2, R3 for rape and B1, B2, B3 for barley) and at 3-stages of Hypericum . . . development (S1: Hypericum pre-germinated seeds, S2: 4-Hypericum leaves developed and S3: formation of branches). Barley and rape residues proved very phytotoxic to root length of Hypericum (-74.51?nd -72.29?espectively). The effects of both crops residues on H. triquetrifolium and root growth were dose dependent and the highest rape residue rate (8 g/pot) caused -67 ?nhibition than control. The crop residues were more phytotoxic to Hypericum in early stages of development. The GC-MS analysis of rape residues identified 10-allelopathic compounds More less

İbrahim İlker ÖZYİĞİT

Article | 2021 | Biotechnology and Biotechnological Equipment35 ( 1 )

Hypericum triquetrifolium Turra is an ecologically, medicinally and economically important species in Tunisia. Thirty-six Hypericum individuals sampled from 6 northern Tunisian locations were investigated for their diversity and relationships using 10 inter-simple sequence repeats (ISSR) markers and 10 morphological features at vegetative stage. The phylogenetic analysis, using 308 bp of sequenced ITS1 region, identified the Hypericum individuals as H. triquetrifolium that clustered with members of genus Hypericum section 9, 9a, 9b and 27, in agreement with the previous molecular classification of the genus. Among the 10 ISSR marker . . .s tested, 7 were scorable and yielded 91 loci with 94.5% of polymorphism. UBC848 and UBC836 were the most polymorphic ISSR markers. The level of genetic diversity (HT = 0.247) and gene flow between the six populations (N (m) = 1.169) were moderate. The structure analysis revealed three genetic subpopulations: individuals of Le Krib location formed a subpopulation divergent from two other subpopulations, probably due to its northwestern and high-altitude geographic barriers, and its sub-humid microclimate. Zaghouan, northeastern location in the lower semi-arid, with the highest genetic (I = 0.370) and morphological (I = 0.631) Shanno More less

Hasan CAN | İbrahim İlker ÖZYİĞİT | Mustafa PAKSOY

Article | 2019 | Journal of Genetics98 ( 3 )

Advanced marker technologies are widely used for evaluation of genetic diversity in cultivated crops, wild ancestors, landraces or any special plant genotypes. Developing agricultural cultivars requires the following steps: (i) determining desired characteristics to be improved, (ii) screening genetic resources to help find a superior cultivar, (iii) intercrossing selected individuals, (iv) generating genetically hybrid populations and screening them for agro-morphological or molecular traits, (v) evaluating the superior cultivar candidates, (vi) testing field performance at different locations, and (vii) certifying. In the cultivar . . . development process valuable genes can be identified by creating special biparental or multiparental populations and analysing their association using suitable markers in given populations. These special populations and advanced marker technologies give us a deeper knowledge about the inherited agronomic characteristics. Unaffected by the changing environmental conditions, these provide a higher understanding of genome dynamics in plants. The last decade witnessed new applications for advanced molecular techniques in the area of breeding, with low costs per sample. These, especially, include next-generation sequencing technologies like reduced representation genome sequencing (genotyping by sequencing, restriction site-associated DNA). These enabled researchers to develop new markers, such as simple sequence repeat and single- nucleotide polymorphism, for expanding the qualitative and quantitative information on population dynamics. Thus, the knowledge acquired from novel technologies is a valuable asset for the breeding process and to better understand the population dynamics, their properties, and analysis methods More less

İbrahim İlker ÖZYİĞİT

Article | 2021 | Desalination and Water Treatment239

In this work, the experiments related to seasonal variations of phytoplankton composition and heavy metal contamination along with some water quality parameters were carried out throughout the channel, serving as a waterway between Lake Kucukcekmece and the Sea of Marmara. Considered as a Class B wetland, the lake has been stayed within the metropolitan area of Istanbul overtime. The samples from the surface water were taken monthly by using Nansen bottles at 5 sampling sites through the period of March-November. After experimental procedures, a total of 46 taxa were recorded belonging to Bacillariophyta (20), Charophyta (4), Chloro . . .phyta (6), Cryptophyta (1), Cyanobacteria (4), Euglenozoa (3), and Miozoa (8) divisions. The phytoplankton densities were found to be varied from 76 individual c More less

İbrahim İlker ÖZYİĞİT

Article | 2020 | Turkish Journal of Chemistry44 ( 4 )

The objective of this study is to synthesize ZnO and Mg doped ZnO (Zn1-xMgxO) nanoparticles via the sol-gel method, and characterize their structures and to investigate their biological properties such as antibacterial activity and hemolytic potential.Nanoparticles (NPs) were synthesized by the sol-gel method using zinc acetate dihydrate (Zn(CH3COO)(2) .2H(2)O) and magnesium acetate tetrahydrate (Mg(CH3COO)(2) .4H(2)O) as precursors. Methanol and monoethanolamine were used as solvent and sol stabilizer, respectively. Structural and morphological characterizations of Zn1-xMgxO nanoparticles were studied by using XRD and SEM-EDX, resp . . .ectively. Photocatalytic activities of ZnO and selected Mg-doped ZnO (Zn1-xMgxO) nanoparticles were investigated by degradation of methylene blue (MeB). Results indicated that Mg doping (both 10?nd 30?to the ZnO nanoparticles enhanced the photocatalytic activity and a little amount of Zn0.90Mg0.10O photocatalyst (1.0 mg/mL) degraded MeB with 99?fficiency after 24 h of irradiation under ambient visible light. Antibacterial activity of nanoparticles versus Escherichia coli (E. coli) was determined by the standard plate count method. Hemolytic activities of the NPs were studied by hemolysis tests using human erythrocytes. XRD data proved that the average particle size of nanoparticles was around 30 nm. Moreover, the XRD results indicated that the patterns of Mg doped ZnO nanoparticles related to ZnO hexagonal wurtzite structure had no secondary phase for x More less

İbrahim İlker ÖZYİĞİT

Article | 2020 | Biotechnology and Biotechnological Equipment34 ( 1 )

Boron (B) is an essential plant micronutrient but studies regarding its transport are still limited to a few plants. This work identified two major B transport sequences in plants, NIP5;1 boric acid channel protein and BOR1 transporter. 80 BOR1 and 34 NIP5;1 homologs were identified in 18 different plant genomes. BOR1 homologs had a HCO3-transporter domain, 649-737 amino-acid residues with mainly basic nature, putative 8-11 transmembrane domains (TMDs) and 11-13 exons. NIP5;1 homologs had a MIP family domain, 294-311 amino-acid residues with basic nature, 5-6 putative TMDs and 3-5 exons. Tyrosine-based motif, acidic di-leucine motif . . . and lysine residue, reported for polarity, vacuolar sorting and B-dependent degradation, were identified in BOR1 homologs. Two NPA motifs and an ar/R selectivity filter with AIGR residues, reportedly essential in B transport, were also found in NIP5;1 homologs. Two NPA motifs in AtNIP5;1 and OsNIP3;1 homologs were NPS and NPV, whereas in sequences homologous to AtNIP6;1 were NPA/V. Besides, ar/R selectivity filters were identified with A(N/S/T)IGR residues in NIP5;1 and NIP3;1 homologs. The BOR1 and NIP5;1 model structures were mainly conserved. Under different perturbations, Arabidopsis thaliana NIP5;1 and NIP6;1 genes demonstrated similar expression patterns although they act in different tissues, suggesting a common regulatory mechanism, whereas BOR1 showed a different expression pattern. BOR1 was substantially expressed in primary root, radicle and flower; NIP5;1 in primary root and roots, and NIP6;1 in petiole. NIP5;1, 6;1 and BOR1 expression in other plant organs implied their involvement in different pathways in addition to B uptake and its mobilization More less

İbrahim İlker ÖZYİĞİT

Article | 2020 | Journal of Natural Fibers19 ( 5 )

WOS:000555623700001

İbrahim İlker ÖZYİĞİT

Article | 2020 | Polish Journal of Environmental Studies29 ( 5 )

Turkish red pine is an evergreen tree species widely distributed in the Aegean and Mediterranean regions in Turkey. In the present work, the heavy metal pollution level in Istanbul was investigated using Turkish red pine as a biomonitor. For determining heavy metal concentrations (Cd, Co, Cr, Mn, Ni and Pb) in leaf (unwashed and washed) and bark samples of Turkish red pine and its co-located soil samples we used ICP-OES. Additionally, particulate matter profiles of the research areas were investigated. The samples were collected from 5 different localities: 4 from the Bosporus region (Yildiz Grove, Fethi Pasha Grove, Baltalimani Gro . . .ve and Mihrabad Grove) and one from Prince Island, also known as Buyukada (as control). According to our measurements, the highest heavy metal accumulations (in mg kg(-1) DW) in plant part samples were measured between 1.526 /- 0.012 and 1.639 /- 0.015 for Cd, 0.543 /- 0.007 and 0.600 /- 0.009 for Co, 5.110 /- 0.064 and 5.648 /- 0.072 for Cr, 9.347 /- 0.105 and 10.331 /- 0.111 for Mn, 7.483 /- 0.091 and 8.271 /- 0.096 for Ni, and 13.848 /- 0.159 and 14.950 /- 0.167 for Pb, while the highest heavy metal accumulations (in mg kg(-1) DW) in soil samples were measured between 1.813 /- 0.021 and 1.974 /- 0.029 for Cd, 6.326 /- 0.082 and 6.992 /- 0.091 for Co, 22.017 /- 0.284 and 23.685 /- 0.301 for Cr, 268.333 /- 3.153 and 297.361 /- 3.529 for Mn, 15.194 /- 0.176 and 16.792 /- 0.193 for Ni, and 68.778 /- 0.715 and 74.514 /- 0.883 for Pb. The highest outdoor particulate matter levels (in mu g/m(3)) in research areas were also determined as 27.103 for fine (PM2.5) and 67.792 for coarse (PM10) aerosols. The findings revealed that Turkish red pine could accumulate noteworthy amounts of heavy metals More less

İbrahim İlker ÖZYİĞİT | Gülbübü KURMANBEKOVA

Article | 2018 | Fresenius Environmental Bulletin27 ( 2 )

Cirsium species have economic importance because of their botanical and medicinal properties. Thus, this study has aimed to investigate some heavy metal (Fe, Mn and Zn) and mineral nutrient (B, Ca, K, Mg and Na) status of narrow endemic Cirisum byzantinum. Plant and soil element concentrations were analyzed by using ICP-OES and the evaluation of plant-soil interactions for heavy metals and mineral nutrients were carried out by statistical methods. The measured element concentrations (in mg/kg) were in ranges of 5.936-19.044 for B, 1862.161-4811.168 for Ca, 12.610-38.474 or Fe, 3421.383-6881.885 for K, 190.984-593.427 for Mg, 3.830-1 . . .7.301 for Mn, 21.236-62.025 for Na, and 6.183-28.060 for Zn in plant parts. Statistical analysis for element interactions between root/leaf and co-located soil samples revealed that there a was positive correlation between B, Ca, Fe, K, Mg, Mn, Na and Zn found in soil and Na and Zn found in roots, and Fe, Mn and Na found in leaves, respectively. Herein, results of this study could provide preliminary knowledge for future studies to better understand element uptake and heavy metal accumulation status of endemic species. Also, this study could also provide some insights for responsible authorities to develop conservation strategies More less